Wednesday, July 22, 2015

RNA-Seq expression metrics: FPKM, RPKM and TMP explained by StatQuest


FPKM / RPKM and TPM are one of the most widely used transcript and gene expression normalization methods in RNA-Seq studies. FPKM and RPKM are similar metrics except the former applies to paired-end sequencing while the latter one is for single-end data. TMP is bit different as it is calculated using a different order of operations. StatQuest explains it well in a nutshell (please follow the video below and read on the following link):



For text summary please refer to: StatQuest page


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